Curriculum vitae

Athanasios-Nasir Shaukat, MSc, PhD

Postdoctoral Fellow, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health, Bethesda, MD.

01

Research experience

Sep 2023 – present

Postdoctoral Fellow

Jinwei Zhang Lab, NIDDK, National Institutes of Health, Bethesda, MD

Structural studies of RNA–protein and RNA–RNA interactions, including ribonucleases and other RNA-binding proteins, using cryo-EM and X-ray crystallography.

2021 – 2023

Researcher, RIBOREGULON

RNA Biology Group, University of Patras · PI: Prof. Constantinos Stathopoulos

Riboswitches as regulators of microbial infection mechanisms and antibiotic resistance.

2020 – 2021

Researcher, RIBOMAP

RNA Biology Group, University of Patras · PI: Prof. Constantinos Stathopoulos

Mapping the role of the human RNase P ribonucleoprotein in transcription and translation regulation using genome-editing tools.

Nov 2017 – Jul 2023

Doctoral Researcher

RNA Biology Group, University of Patras

Human RNase Z (ELAC1, ELAC2) and RNase P: CRISPR-Cas9 knockouts, RNA-seq and CLIP-seq revealing ELAC1 as a processing enzyme for vault RNAs and other non-coding RNAs.

02

Education

2017 – 2023

PhD, Biochemistry and Molecular Biology

School of Medicine, Department of Biochemistry, University of Patras, Greece

Supervised by Prof. Constantinos Stathopoulos.

2015 – 2017

MSc, Biomedical Sciences

School of Medicine, Department of Biochemistry, University of Patras, Greece

Specialisation in Medical Biochemistry–Immunology.

2011 – 2015

BSc, Biology

Department of Biology, University of Patras, Greece

03

Skills

Structural biology

  • Cryo-EM sample prep & grid optimisation
  • Cryo-EM data collection & processing
  • X-ray crystallography
  • Structure determination

Biochemistry

  • Recombinant protein expression
  • Affinity chromatography
  • In vitro transcription
  • Enzyme kinetics

Molecular & cell biology

  • Molecular cloning
  • qRT-PCR
  • RNA-seq / NGS analysis
  • CRISPR-Cas9
  • TALENs
  • CRISPRi
  • Cell culture & transfection

Immunology

  • PBMC isolation
  • CD3⁺ T-cell isolation & stimulation
  • Western blotting

Other

  • Radioisotope handling

04

Honours

2026

Fellows Award for Research Excellence (FARE 2027)

National Institutes of Health · Office of Intramural Training & Education — Study Section: DNA Replication, Damage and Repair

2021

Oral Presentation Award

71st Conference of the Hellenic Society of Biochemistry and Molecular Biology

2021

Travel Grant

71st Conference of the Hellenic Society of Biochemistry and Molecular Biology

2019

Travel Grant

Onassis Foundation Science Lecture Series — Genome Editing

2015 – 2019

Bodossaki Foundation Scholarship

Postgraduate studies

05

Teaching

2022 – 2023

Instructor

Patras Public Experimental Vocational Training Institute

Biotechnology I · Parasitology · Fundamentals of the Microbiology Lab

2015 – 2022

Teaching Assistant

School of Medicine, University of Patras

Biochemistry I, II and III laboratory practicals for medical students

06

Memberships

2023 – present

RNA Society

2017 – 2023

Hellenic Society of Biochemistry and Molecular Biology (HSBMB)

2017 – 2023

Federation of European Biochemical Societies (FEBS)

07

Languages

Native

Greek

Full professional proficiency

English

08

Publications

13 journal articles and reviews and 1 preprint — see the full list.